Fig. 6

Metformin attenuates subchondral osteosclerosis in the destabilization of the medial meniscus mice model. A Sagittal plane, B coronal plane and C transaxial plane of μ-CT two-dimensional reconstruction, blue boxes indicating areas of osteosclerosis. E BV/TV, F Tb. Sp and G BMD. Immunofluorescence staining of D Runx2 and H the proportion of Runx2-positive cells (scale bar, 100 µm). One-way ANOVA and Tukey’s multiple comparison tests were used to compare the data. *P < 0.05; **P < 0.01; ***P < 0.001