Fig. 5

Effects of HDAC1 on inflammation and osteogenic differentiation of AS fibroblasts. A Cells viability was detected by CCK-8 assay. B IL-6 expression in AS fibroblasts was detected by ELISA. C IL-1β expression in AS fibroblasts was detected by ELISA. D TNF-β expression in AS fibroblasts was detected by ELISA. (E) ALP activity of AS fibroblasts. F Mean fluorescence intensity of calcium ions. G Intracellular calcium concentration was detected by flow cytometry. H Representative images showing Alizarin red staining. Magnification, × 100. Data were shown as mean ± SD. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001, compared with the AAV-NC group. ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001, compared with the AAV-HDAC1 group