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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Circ-FOXM1 promotes the proliferation, migration and EMT process of osteosarcoma cells through FOXM1-mediated Wnt pathway activation

Fig. 4

Circ-FOXM1 targeted miR-320a and miR-320b to modulate FOXM1. a The Venn diagram of miRNAs binding to FOXM1 from four databases (PITA, miRanda, TargetScan and miRmap) included in ENCORI website. b Luciferase reporter assay was utilized to screen out the miRNA targeting FOXM1. c, d The mRNA and protein levels of FOXM1 were detected through qRT-PCR and western blot when miR-320a and miR-320b were overexpressed in OS cells. e ENCORI predicted the binding sites between miR-320a or miR-320b and FOXM1. f Luciferase reporter assay was applied for proving the binding site of miR-320a or miR-320b and FOXM1. g ENCORI predicted the binding sites between miR-320a or miR-320b and circ-FOXM1. h Luciferase reporter assay was applied for proving the binding site of miR-320a or miR-320b and circ-FOXM1. i RIP assay tested the enrichment of miR-320a, miR-320b, circ-FOXM1 and FOXM1 in Ago2-RISCs. **P < 0.01

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