Table 2 Primer sequences for qRT-PCR
Targets | Sequences (5′–3′) |
|---|---|
miR-181a-5p-F-hsa | CCGCGAACATTCAACGCTGTCG |
miR-181a-5p-R-hsa | ATCCAGTGCAGGGTCCGAGG |
U6-F-F-hsa | CAAATTCGTGAAGCGTTCCATAT |
U6-R-F-hsa | GCTTCACGAATTTGCGTGTCATCCTTGC |
miR-181a-5p-F-mus | GGGAACATTCAACGCTGTCG |
miR-181a-5p-R-mus | GTGCGTGTCGTGGAGTCG |
U6-F-F-mus | GCTTCGGCAGCACATATACTAAAAT |
U6-F-R-mus | CGCTTCACGAATTTGCGTGTCAT |
Gm37494-F-mus | GGAGATTCCTAAGAA |
Gm37494-R-mus | GACTTTGTTGCTGTA |
GABRA1-R-hsa | ATGCGGATTTCGTCCTGACT |
GABRA1-R-hsa | GCCTCGAGCTCCATCATTCT |
GABRA1-F-mus | GAGGGTATGCGTGGGATG |
GABRA1-R-mus | GCTTGACTTCTTTCGGTTCTAT |
GAPDH-F-mus | CCCTTAAGAGGGATGCTGCC |
GAPDH-R-mus | ACTGTGCCGTTGAATTTGCC |
GAPDH-F-hsa | AATGGGCAGCCGTTAGGAAA |
GAPDH-R-hsa | GCGCCCAATACGACCAAATC |