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Fig. 6 | Journal of Orthopaedic Surgery and Research

Fig. 6

From: Long non-coding RNA Gm37494 alleviates osteoarthritis chondrocyte injury via the microRNA-181a-5p/GABRA1 axis

Fig. 6

Gm37494 relieves IL-1β-induced chondrocyte damage by increasing GABRA1 expression. Oe-Gm37494 and sh-GABRA1 were simultaneously transfected into 1L-1β-treated chondrocytes. A qRT-PCR and western blot detection of the levels of GABRA1 mRNA and the encoded protein GABAARα1, respectively, in chondrocytes induced by IL-1β; B–C CCK-8 (B) and EdU (C) assays of the proliferation of IL-1β-induced chondrocytes; D ELISA determination of the levels of inflammatory factors TNF-α, IL-6, and IL-10; E flow cytometry measurement of chondrocyte apoptosis; F western blot analysis of the expression of apoptotic proteins Bax, Bcl-2, and cleaved caspase 3 in chondrocytes induced by IL-1β. Data were expressed as mean ± standard deviation, and each experiment was repeated 3 times. *P < 0.05, compared with the oe-NC + sh-NC group; #P < 0.05, compared with the oe-Gm37494 + sh-NC group

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