Fig. 3

Gm37494 directly binds to and is inversely correlated with miR-181a-5p. A the binding site and mutant sequence between miR-181a-5p and Gm37494; B–C the expression of miR-181a-5p in patients with OA (B) and the mouse model of OA (C) determined by qRT-PCR. D–E the binding between miR-181a-5p and Gm37494 assessed by dual-luciferase reporter gene (D) and RIP assays (E); F the relative expression of miR-181a-5p observed by qRT-PCR after transfection of oe-Gm37494 in chondrocytes induced by IL-1β. Data were expressed as mean ± standard deviation. N = 20 for clinical experiments, and N = 10 mice/group for animal experiments. Cell experiments were repeated 3 times. *P < 0.05, compared with the control or sham group; ^#P < 0.05, compared with the mimic NC, IgG, or IL-1β + oe-NC group