Skip to main content
Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: Long non-coding RNA Gm37494 alleviates osteoarthritis chondrocyte injury via the microRNA-181a-5p/GABRA1 axis

Fig. 2

Overexpression of Gm37494 in vitro remarkably inhibits chondrocyte damage induced by IL-1β. IL-1β-induced chondrocytes were transfected with oe-Gm37494. A Gm37494 expression measured by qRT-PCR in IL-1β-induced chondrocytes; B–C CCK-8 (B) and EdU (C) assays to detect chondrocyte proliferation; D ELISA to detect the levels of inflammatory factors TNF-α, IL-6, and IL-10 in IL-1β-induced chondrocytes; E flow cytometry to examine chondrocyte apoptosis; F western blot to test the expression of apoptotic proteins Bax, Bcl-2, and cleaved caspase 3 in IL-1β-induced chondrocytes. Data were expressed as mean ± standard deviation, and each experiment was repeated 3 times. *P < 0.05, compared with the control group; #P < 0.05, compared with the IL-Iβ + oe-NC group

Back to article page