Fig. 3

miRNA-4701-5p negatively regulates HMGA1 expression in CHON-001 cells. CHON-001 cells were transfected with mimic control, miRNA-4701-5p mimic, control-plasmid, HMGA1-plasmid for 24 h, respectively. The expression and transcription levels of HMGA1 were determined using western blot assay and qRT-PCR, respectively. A The expression of miRNA-4701-5p was determined by qRT-PCR. B The transcription levels of HMGA1 were determined by qRT-PCR, respectively. C, D The protein expression and transcription levels of HMGA1 were determined using western blot assay and qRT-PCR, respectively. **p < 0.01 versus mimic control; ^##p < 0.01 versus control-plasmid; ^&&p < 0.01 versus miRNA-4701-5p mimic + control-plasmid