Cyclic mechanical strain with high-tensile triggers autophagy in growth plate chondrocytes

Table 1 Primers sequence for real-time reverse transcriptase polymerase chain reaction (RT-PCR)

Gene	NCBI version	Length (bp)	Sequence (5′–3′)	Product length (bp)
GAPDH	NM_017008.4	21	AACGACCCCTTCATTGACCTC	85
		21	CCTTGACTGTGCCGTTGAACT
ATG5	NM_001014250.1	22	GTGTGAAGGAAGCTGACGCTTT	144
		21	GGAGGGTATTCCATGAGTTTC
BECN1	NM_053739.2	23	AACTGGACACGAGCTTCAAGATC	78
		22	CCTGGGCTGTGGTAAGTAATGG
LC3B	NM_022867.2	20	CATGCCGTCCGAGAAGACCT	70
		20	GATGAGCCGGACATCTTCCACT
ULK1	NM_001108341.1	19	TGGAGGTGGCCGTCAAATG	202
		20	CGCATAGTGTGCAGGTAGTC

ISSN: 1749-799X