Fig. 6
From: Cyclic mechanical strain with high-tensile triggers autophagy in growth plate chondrocytes
F-actin cytoskeleton mediated CTS-induced autophagy. After receiving CTS (5500 με, 2.0 Hz) for 2 h, chondrocytes were harvested for western blotting or stained with Actin-tracker (green), LC3-antibody (red) and DAPI (blue) to visualize F-actin fibers, LC3 puncta and nuclei by confocal laser scanning microscopy. A, B The protein levels of LC3-II and LC3-I were measured by western blotting. C, D Chondrocytes from control group, strained chondrocytes without cytoD treatment, unstrained chondrocytes with cytoD (1 μM) treatment and strained chondrocytes with cytoD treatment. Scale bar = 10 μm. Error bars represent standard deviations. n = 3, *P < 0.05, **P < 0.005