Fig. 6

Knockdown of HuR or LRP6 repressed cell viability in MC3T3-E1 cells overexpressing Wnt3a. A Representative experimental image (a) and the corresponding quantitative analysis (b) in western blot measurement of Wnt3a expression, the activation of the Wnt pathway detected with TOPFLASH reporter gene assay (c) and the cell viability in MC3T3-E1 cells stably transfected with oe-Wnt3a as detected with MTT assay (d). B Detection of HuR and LRP6 expression by western blot assay in MC3T3-E1 cells transfected with siHuR or siLRP6. C Detection of Wnt pathway activation and cell viability in MC3T3-E1 cells by dual-luciferase reporter assay and MTT assay. D Flow cytometric analysis of cell cycle distribution in MC3T3-E1 cells. *p < 0.05 versus the Vector or NC groups. Measurement data were expressed as mean ± standard deviation. Data between two groups were compared using unpaired t-test. Data comparison among multiple groups was conducted by one-way ANOVA, followed by Tukey’s post hoc test. The cellular experiment was repeated three times independently