Fig. 5

HuR enhanced MC3T3-E1 cell osteogenic differentiation and arrested its apoptosis via modulating LRP6. a RT-qPCR measurement of osteoblast-related marker expression in MC3T3-E1 cells in response to siHuR alone or in combination with LRP6 overexpression or LiCl. b Detection of ALP activity in MC3T3-E1 cells by p-nitrophenol assay in response to siHuR alone or in combination with LRP6 overexpression or LiCl. c Detection of MC3T3-E1 cell viability by MTT assay in response to siHuR alone or in combination with LRP6 overexpression or LiCl. d Detection of the number of calcium nodules in MC3T3-E1 cells by Alizarin red S staining in response to siHuR alone or in combination with LRP6 overexpression or LiCl. e Western blot measurement of HuR, LRP6 and osteogenesis-related proteins (Runx2 and Osterix) in response to siHuR alone or in combination with LRP6 overexpression or LiCl. f Detection of apoptosis of MC3T3-E1 cells by TUNEL staining in response to siHuR alone or in combination with LRP6 overexpression or LiCl. *p < 0.05 versus the NC group, ^#p < 0.05 versus the siHuR + Vector or siHuR + DMSO groups. Measurement data were expressed as mean ± standard deviation. Data comparison among multiple groups was conducted by one-way ANOVA, followed by Tukey’s post hoc test. The cellular experiment was repeated three times independently