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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Potential of RNA-binding protein human antigen R as a driver of osteogenic differentiation in osteoporosis

Fig. 4

HuR stabilized LRP6 mRNA and enhanced its translation. a Detection of LRP6 expression in MC3T3-E1 cells with HuR silencing by western blot assay. b Detection of LRP6 mRNA expression in MC3T3-E1 cells with HuR silencing by RT-qPCR. c Detection of LRP6 mRNA stability in response to HuR knockdown detected by half-life assay. d In vitro translation detection of LRP6 protein synthesis after HuR knockdown. E, Detection of LRP6 mRNA fragments by ribosome translation. f Binding of HuR to 3’-UTR of LRP6 mRNA as validated by dual-luciferase reporter assay. * p < 0.05 versus the NC group. Measurement data were expressed as mean ± standard deviation. Data between two groups were compared using unpaired t-test. The cellular experiment was repeated three times independently

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X