Fig. 4

Circ_0001017 sponged miR-145-5p. A Schematic presentation of the putative binding sites of three miRNAs with respect to circ_0001017. B Quantitative real-time PCR was used to determine the expression level of miR-145-5p in U2OS cells after transfection with miR-145-5p mimics. C Luciferase reporter assay in U2OS cells co-transfected with miR-145-5p mimics or miR-NC and reporter constructs carrying wild-type or mutant miR-145-5p binding sequence of 3′-UTR of circ_0001017. **p < 0.01, ***p < 0.001, compared with miR-NC. D RIP assay was conducted to examine the enrichment of miR-145-5p and circ_0001017 in the Ago2 complex. ***p < 0.001, compared with anti-IgG. E Quantitative real-time PCR was performed to determine miR-145-5p levels in U2OS cells in response to circ_0001017 overexpression or knockdown. **p < 0.01, compared with Vector; ###p < 0.001, compared with si-NC