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Fig. 7 | Journal of Orthopaedic Surgery and Research

Fig. 7

From: Circ_0001174 facilitates osteosarcoma cell proliferation, migration, and invasion by targeting the miR-186-5p/MACC1 axis

Fig. 7Fig. 7

Circ_0001174 could regulate MACC1 expression by sponging miR-186-5p. A The Venn diagram was contained 3 databases (Targetscan, DIANA tools and miRDB) predicted binding mRNAs and differentially expressed mRNAs. B The binding site between miR-186-5p and MACC1. C, D The expression of MACC1was significantly increased in OS tissues (16 pairs of OS and adjacent tissue) and MG63 cells. E The negative correlation was found between miR-186-5p and MACC1. F After transfected with miR-186-5p mimic, the expression of MACC1 was detected by qRT-PCR and western blot, respectively. G qRT-PCR and western blot analysis showed miR-186-5p inhibition could rescue suppressed effect caused by si-circ_0001174 in the expression of MACC1. Results represented the mean ± s.d. of three independent experiments and the relative expression levels of each gene were analyzed using the 2−△△Ct method. ** represent P < 0.01

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X