Fig. 2
From: MiR-539-3p inhibited chondrogenic differentiation in human adipose stem cells by targeting Sox9
MiR-539-3p regulated ASC chondrogenic differentiation. hASCs were transfected with miR-539-3p mimic or miR-539-3p inhibitor, which were cultured in chondrogenic differentiation medium. hASCs cultured in high-glucose DMEM with 1% fetal bovine serum (FBS) were used as control. A–C The expression levels of miR-539-3p in hASCs or chondrogenic differentiation hASCs (CD-ASCs) were detected by RT-qPCR. Gene expression levels of COL2A1 and ACAN in hASCs or CD-ASCs were determined by RT-qPCR (D and E) and western blot (F–H). β-actin expression levels were detected as an endogenous control. **P < 0.01 (VS. Day 0/NC mimic/ASCs), ##P < 0.01 (VS. CD-ASCs). (I) Immunofluorescence assay of Type II collagen. Type II collagen fluorescence (green) and DAPI (blue). Scale bar: 100 μm and 50 μm. The experiment was repeated at least 3 times independently. The data are presented as mean ± standard deviation (SD)