Fig. 3

miR-206 targeted GJA1. A–C miR-1-3p, miR-613 and miR-206 expression were detected by RT-PCR in clinical IDD tissues and normal intervertebral disk tissues. D Correlation analysis was performed between miR-206 and GJA1 in IDD tissues. E Binding sites of miR-206 and GJA1 were predicted through Targetscan_7.1 (TargetScanHuman 7.1). F The relationship between miR-206 and GJA1 was verified by luciferase reporter gene. G NP cells were transfected with inhibitor NC, miR-206 inhibitor, mimics NC, or miR-206 mimics, the expression of miR-206 was detected by RT-PCR. I GJA1 expression was measured by western blot. **P < 0.01 versus control group, **P < 0.01 versus mimics NC group, ##P < 0.01 versus inhibitor NC group