Fig. 5

miR-671-5p interacts with the 3′UTR of RTN3. A The predicted binding sequence with miR-671-5p in RTN3 was predicted by starbase database. B Dual-luciferase reporter assay was conducted to validate the target interaction between miR-671-5p and RTN3. C and D The effect of miR-671-5p overexpression or knockdown on the expression of RTN3 protein was determined by Western blot assay. E The protein level of RTN3 was measured in chondrocytes treated with IL-1β (10 ng/mL, 24 h) by Western blot assay. F Chondrocytes were transfected with circ_0043947-si#1 alone or together with anti-miR-671-5p, and the protein level of RTN3 was measured by Western blot assay. G The overexpression efficiency of RTN3 plasmid in chondrocytes was determined by Western blot assay. H Chondrocytes were transfected with miR-671-5p alone or together with RTN3 plasmid, and the protein expression of RTN3 was measured by Western blot assay. ***P < 0.001