Fig. 5

MiR-30b-3p can target ELK1. A The binding sites between miR-30b-3p and ELK1 were predicted by starbase2.0. B Relative luciferase activity of ELK1 vector was detected by DLR assay. **P < 0.01, versus miR-NC. C qRT-PCR was used to detect relative expression of ELK1 in osteosarcoma tissues and adjacent normal tissues. P < 0.001, versus adjacent normal tissues. D Correlation of ELK1 and miR-30b-3p expression in osteosarcoma tissues was analyzed by Pearson's correlation analysis. P = 0.0279, r =  − 0.2939. E After transfection with miR-30b-3p mimics or miR-NC in MG63 and U2OS cells, expression of ELK1 was detected by western blot. **P < 0.01, versus miR-NC. F After transfection with sh-LINC00662 or sh-NC in MG63 and U2OS cells, protein level of ELK1 was measured by western blot. **P < 0.01, versus sh-NC