Fig. 2

Knockdown of lncRNA LINC00662 represses proliferation, migration and invasion of osteosarcoma cells. A Relative expression of LINC00662 in osteosarcoma cell lines was detected by qRT-PCR. **P < 0.01, versus hFOB. B Relative expression of LINC00662 in U2OS and MG63 cell lines after transfection with sh-LINC00662 was detected by qRT-PCR. **P < 0.01, versus sh-NC. C The cell viability in U2OS and MG63 cell lines was detected by MTT assay. *P < 0.05, **P < 0.01, versus sh-NC. D Wound healing rate was detected by wound healing assay. **P < 0.01, versus sh-NC. Scale bar = 100 µm. E Relative number of invasion cells was detected by transwell assay. **P < 0.01, versus sh-NC. Scale bar = 100 µm. F The solid tumor image and tumor volume in xenograft mice after injection of sh-NC or sh-LINC00662. *P < 0.05, **P < 0.01, versus sh-NC. G Tumor weight in xenograft mice after injection of sh-NC or sh-LINC00662. **P < 0.01, versus sh-NC. H Immunohistochemistry (IHC) staining of Ki-67 in xenograft mice generated by sh-NC or sh-LINC00662 injection. magnifications, × 200