Table 1 The grouping of experiments (Exp)
Group | Treatment | |
|---|---|---|
Exp. I | Control group | Osteogenic induction medium |
10 μM group | Osteogenic induction medium + 10 μM JRL extract | |
20 μM group | Osteogenic induction medium + 20 μM JRL extract | |
40 μM group | Osteogenic induction medium + 40 μM JRL extract | |
80 μM group | Osteogenic induction medium + 80 μM JRL extract | |
Exp. II | Blank group | DMEM medium |
OIM group | Osteogenic induction medium + PBS | |
OIM + 20 μM group | Osteogenic induction medium + 20 μM JRL extract | |
OIM + 40 μM group | Osteogenic induction medium + 40 μM JRL extract | |
Exp. III | Blank group | DMEM medium |
OIM group | Osteogenic induction medium + PBS | |
OIM + 20 μM group | Osteogenic induction medium + 20 μM JRL extract | |
OIM + 20 μM group + Noggin | Osteogenic induction medium + 20 μM JRL extract + 100 ng/mL Noggin | |
OIM + 20 μM group + DKK1 | Osteogenic induction medium + 20 μM JRL extract + 2 mg/mL DKK1 |