Fig. 5

MiR-671-5p directly targeted STAT3. A The binding sites between STAT3 and miR-671-5p. B The association between miR-671-5p and STAT3 was verified by dual-luciferase reporter assay. C, D The mRNA and protein levels of STAT3 in RA and normal synovial tissues were measured via qRT-PCR and western blot, respectively. E The linear correlation between STAT3 and miR-671-5p in RA synovial tissues was analyzed. F The protein level of STAT3 in RA-FLSs and normal FLSs was measured by western blot. G The expression of miR-671-5p in RA-FLSs transfected with anti-miR-NC or anti-miR-671-5p was detected by qRT-PCR. H The protein level of STAT3 in RA-FLSs transfected with miR-NC, miR-671-5p, anti-miR-NC or anti-miR-671-5p was measured by western blot. ***P < 0.001, ****P < 0.0001