Fig. 3

TAA promoted osteoclastogenesis in BMMs. A Representative images of immunofluorescent microscopy showing the TARP (green) and nuclear (blue) in RANKL and M-CSF-treated or TAA-treated osteoclasts. C Effects of TAA at different concentrations (0, 0.5, 1 mg/mL) on the formation of osteoclast-like cells derived from BMMs. B, D The number of osteoclasts showed TRAP-positive multinucleated cells (B for Immunofluorescence, D for TRAP staining). Values are expressed as mean ± SD of three individual experiments; *P < 0.05, **P < 0.01 and ***P < 0.001 versus control; ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 versus RANKL and M-CSF