Fig. 2

Effects of TAA on BMMs proliferation and viability. A Chemical structure of TAA. B Effects of TAA on the cell viability of BMMs. Cells were treated with the indicated concentrations of TAA for the indicated times. Cell viability was determined by the CCK-8 assay. Dates are expressed as mean ± SD. C BMMs were treated with the indicated concentrations of TAA for 24 h, then, cells were labeled for 15–20 min at 37 °C with 3 μM carboxyfluorescein diacetate succinimidyl ester (CFSE). Cell fluorescence was evaluated by flow cytometry. D The bar chart represented cells divided index. E Cell cycle distribution was measured in BMMs by flow cytometry analysis after TAA was treated with indicated concentrations for 24 h. F The bar chart represented the percentage of cells in G0/G1, S or G2/M phase, as indicated. Values are expressed as mean ± SD of three individual experiments; *P < 0.05 and ** P < 0.01 versus control