Fig. 5
Effects of HIF-1α inhibitor on HIF-1α activity and the tenogenic differentiation of ADSCs. A, B The ADSCs supplemented with 2-MoeE2 at indicated concentrations. The HIF-1α and SCX expression were detected with real-time PCR analysis by concentration gradient test.*p ˂ 0.05, compared with control. C–F The ADSCs were treated with 10 μM of 2-MoeE2 under normoxia or hypoxia for 24 h, the expression levels of (C) HIF-1α, (D) TNMD, (E) DCN, and (F) SCX were assessed by RT-PCR. All results were presented as mean ± SD. *p ˂ 0.05, compared with the 20% O2 culture group without 2-MoeE2 treatment; #p ˂ 0.05, compared with the 5%O2 culture group without 2-MoeE2 treatment