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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: miR-653-5p suppresses the viability and migration of fibroblast-like synoviocytes by targeting FGF2 and inactivation of the Wnt/beta-catenin pathway

Fig. 3

FGF2 is the direct target of miR-653-5p. A The complementary sequences of miR-653-5p and corresponding sequence of the 3'‑UTR of FGF2 was predicted by starBase website. B Luciferase reporter assay showed luciferase activity of FGF2-WT or FGF2-MUT in HFLS-RA cells transfected with NC mimics or miR-653-5p mimics. C Association between miR-653-5p and FGF2 was determined using an RNA pull-down assay. D RT‑qPCR analysis was used to evaluate the expression of FGF2 in HFLS-RA cells transfection of miR-653-5p mimics or NC mimics and miR-653-5p inhibitor or NC inhibitor. **P < 0.01, ***P < 0.001

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