Fig. 2

The addition of miR-653-5p restrains the viability, migration, and invasion in HFLS-RA cells. A RT-qPCR analysis showed the expression of miR-653-5p in HFLS-RA cells transfection of miR-653-5p mimics or NC mimics and miR-653-5p inhibitor or NC inhibitor. B and C MTT assay showed cell viability in HFLS-RA cells transfection of miR-653-5p mimics or NC mimics and miR-653-5p inhibitor or NC inhibitor. D and E HFLS-RA cell migration and invasion were measured using wound healing and transwell assays after transfection with miR-653-5p mimics or NC mimics and miR-653-5p inhibitor or NC inhibitor. F and G The protein and mRNA expression of Rac1, Cdc42, and RhoA in HFLS-RA cells transfection of miR-653-5p mimics or NC mimics and miR-653-5p inhibitor or NC inhibitor was detected by western blot and RT-qPCR assay. *P < 0.05, **P < 0.01, ***P < 0.001