Fig. 3

The effects between Rg3 and circ_0003074 overexpression on OS cell malignancy. A The efficiency of circ_0003074 overexpression was determined by qRT-PCR. B–I Both MG63 cells and Saos2 cells were treated with 80 µM Rg3, pCD-ciR, circ_0003074, 80 µM Rg3 + pCD-ciR or 80 µM Rg3 + circ_0003074, with untreated cells as a control, and cell viability was investigated by CCK-8 (B), cell proliferation by EdU assay (C), cell apoptosis by flow cytometry analysis (D, E), cell migration by wound-healing assay (F), cell invasion by transwell invasion assay (G), and the protein expression of PCNA, cleaved caspase-3 and MMP9 by Western blotting (H, I). *P < 0.05, **P < 0.01 and ***P < 0.001