Fig. 1

The expression of circ_0003074 in Rg3-treated MG63 and Saos2 cells. A The schematic illustration showing the generation of circ_0003074. B, C Circ_0003074 expression was detected by qRT-PCR in OS tissues (N = 38), adjacent normal tissues (N = 38), hFOB1.19 cells, MG63 cells and Saos2 cells. D–G Circ_0003074 stability was identified by RNase R and Actinomycin D treatment assays. H, I Circ_0003074 expression was determined by qRT-PCR in OS cells treated with Rg3 (0, 40, 80 and 120 µM). *P < 0.05, **P < 0.01 and ***P < 0.001