Fig. 5

ATG14 is a target of miR-338-3p. A The putative binding site between ATG14 and miR-338-3p. B, C Dual-luciferase reporter assay for the luciferase activity of wild and mutated ATG14 reporter after miR-338-3p overexpression in H929 and MM.1S cells. D, E Anti-Ago2 RIP assay was used in H929 and MM.1S cells to determine ATG14 and miR-338-3p RNA enrichment in immunoprecipitate complexes. F, G RT-qPCR and Western blot analysis of ATG14 expression level in bone marrow aspirates of MM patients and normal healthy donors. H Western blot analysis of ATG14 expression level in MM cell lines (H929, OPM2 and MM.1S) and normal nPCs cells. I Western blot analysis of ATG14 protein level in H929 and MM.1S cells transfected with ATG14 or vector. J, K Western blot analysis of ATG14 protein level in H929 and MM.1S cells transfected with miR-NC, miR-338-3p, miR-338-3p + vector, or miR-338-3p + ATG14. *P < 0.05