|
Target gene
|
Primer sequence (5′–3′)
|
|---|
|
Mouse COL1 a1
|
F: GACGCCATCAAGGTCTACTG
|
|
R: ACGGGAATCCATCGGTCA
|
|
Mouse COL1 a2
|
F: GGAGGGAACGGTCCACGAT
|
|
R: GAGTCCGCGTATCCACAA
|
|
Mouse DDR2
|
F: CTCCCAGAATTTGCTCCAG
|
|
R: GCCACATCTTTTCCTGAGA
|
|
Mouse P38
|
F: GGATATTTGGTCCGTGGGCT
|
|
R: CCGTCAGACGCATTATCTGC
|
|
Mouse OPN
|
F: CCAGCCAAGGACCAACTACA
|
|
R: AGTGTTTGCTGTAATGCGCC
|
|
Mouse BSP
|
F: AGAAAGAGCAGCACGGTTGA
|
|
R: AATCCTGACCCTCGTAGCCT
|
|
Mouse OCN
|
F: ATTGTGACGAGCTAGCGGAC
|
|
R: TCGAGTCCTGGAGAGTAGCC
|
|
Mouse GAPDH
|
F: CCTGCACCACCAACTGCTTA
|
|
R: CATCACGCCACAGCTTTCCA
|
- COL1 Collagen 1; DDR2 Disc protein domain receptor 2; P38 P38 mitogen‐activated protein kinase; BSP bone sialoprotein; OCN osteocalcin; F forward; R reverse; RT‐qPCR reverse transcription-quantitative polymerase chain reaction
