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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Discoidin domain receptor 2 activation of p38 mitogen-activated protein kinase as an important pathway for osteonectin-regulating osteoblast mineralization

Fig. 5

DDR2-siRNA/DDR2-blocker suppressed the osteogenic activity. The cell ultrastructure of osteoblasts was observed by TEM in response to the treatment of CON, ON, Inhibitor, and DDR2-siRNA group; DDR2-siRNA/inhibitor group both showed fewer organelles and intracellular vesicles. Bars: a, c, e and g, 20 µm; Bars: b, d, f and h, 10 µm; N, nucleus; m, mitochondria; ly, lysosomes; mb, multivesicular bodies; iv, intracellular vesicle; TEM, transmission electron microscopy; CON group: control group, the osteoblasts were added with mineralized solution; ON group: osteonectin group, the osteoblasts were added with mineralized solution + 1 µg/mL osteonectin; Inhibitor group: the osteoblasts were added with mineralized solution + 1 µg/mL osteonectin + 230 nM WRG-28; DDR2-siRNA group: the osteoblasts were transfected with DDR2-siRNA and added with mineralized solution + 1 µg/mL osteonectin

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X