Fig. 4

DDR2-siRNA/DDR2-blocker reduced the formation of mineralized nodules. A Calcium nodules (blue circle) observation stained with alizarin red under a microscope (× 100) after induced mineralization for 14 days in response to the treatment of CON, ON, Inhibitor and DDR2-siRNA group; B the Adobe Photoshop CS5 system was used to calculate the positive staining of calcium nodules; C quantitative analysis of calcium nodules in each group. Bars, (a) 200 µm, *P < .05, **P < .01, ***P < .001 versus the Con group; #P < .05 versus the ON group; three samples in each group were observed; data were expressed by means ± standard deviation (SD); CON group: control group, the osteoblasts were added with mineralized solution; ON group: osteonectin group, the osteoblasts were added with mineralized solution + 1 µg/mL osteonectin; Inhibitor group: the osteoblasts were added with mineralized solution + 1 µg/mL osteonectin + 230 nM WRG-28; DDR2-siRNA group: the osteoblasts were transfected with DDR2-siRNA and added with mineralized solution + 1 µg/mL osteonectin