Fig. 2
From: GTSE1 is possibly involved in the DNA damage repair and cisplatin resistance in osteosarcoma
GTSE1 promotes S/G2 transition and DNA replication in OS cells. A mRNA expression of GTSE1 in MG-63 and 143B cells after sh-GTSE1 or oe-GTSE1 transfection, respectively, examined by RT-qPCR (unpaired t test); B, C cell cycle progression in MG-63 (B) and 143B (C) cells determined by flow cytometry (two-way ANOVA); D–E, protein levels of Cyclin D1, Cyclin E1, and PCNA in MG-63 (D) and 143B (E) cells determined by western blot analysis (two-way ANOVA); F, G DNA replication ability of MG-63 (F) and 143B (G) cells examined by EdU labeling assay (unpaired t test). Data were presented as the mean ± SD from three repetitions. *p < 0.05, **p < 0.01