Fig. 4

Circ-ATAD1 overexpression suppressed miR-154-5p maturation in Hs 3.T and MG-63 cells. Hs 3.T and MG-63 cells were transfected with circ-ATAD1 expression vector or miR-154-5p mimic, and circ-ATAD1 and miR-154-5p overexpression was confirmed every 24 h until 72 h (A). The effects of circ-ATAD1 overexpression on the levels of mature miR-154-5p (B) and premature miR-154-5p (C) as well as the expression of circ-ATAD1 in cells with miR-154-5p overexpression (D) were analyzed by RT-qPCR. *, p < 0.05; **, p < 0.01; ***, p < 0.001