Fig. 4
From: Investigation of the role of the miR17-92 cluster in BMP9-induced osteoblast lineage commitment
RB1 is essential for miR-17 effect on osteogenesis in vivo and in vitro. A RB1 rescued BMP9-induced ALP activity in miR-17 iMEFs. Qualitative histochemical staining (a) and Quantitative bioluminescence assay (b). **p < 0.01. B RB1 rescued BMP9-induced calcium deposit in miR-17 iMEFs. C Adenovirus adBMP9 overexpression in stable cell lines. D H & E (a) and Masson’s Trichrome staining (b) of the bone masses retrieved at week 4. Representative images are shown. B, mature bone; M, undifferentiated MSCs