Fig. 1
From: Investigation of the role of the miR17-92 cluster in BMP9-induced osteoblast lineage commitment
Expression levels of individual member of miR-17-92 and osteogenesis markers in BMP9-induced MSCs. A The expression levels of mouse miR-17, miR-18a, miR-20a and miR-92a-1 in BMP9-induced iMEFs at the indicated time points. B The expression levels of osteogenesis markers OPN and OCN at the indicated time points. Samples were normalized with the reference gene gapdh. Each assay condition was done in triplicate. **p < 0.01, *p < 0.05. Runx2, runt-related transcription factor 2; OCN, osteocalcin. C Stable cell lines expressed individual member of miR-17-92 cluster. (a) Blank fields of stable cell lines. (b) Fluorescence fields of stable cell lines. D qPCR detected the expression of individual member of miR-17-92 cluster. All samples were normalized with the reference gene gapdh. Each assay was done in triplicate. *p < 0.05, **p < 0.01