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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: Protective effects of low-magnitude high-frequency vibration on high glucose-induced osteoblast dysfunction and bone loss in diabetic rats

Fig. 2

Effects of LMHFV on the differentiation and mineralization of HG-induced MC3T3-E1 cells. A BCIP/NBT staining was performed to assess the ALP activity of the respective groups. B The ALP activity of each group was quantitatively analysed by using an ALP activity kit. The ALP activity was reflected by the absorbance of lysate at 405 nm, and the results are illustrated in the bar graph. C Alizarin Red S staining was performed to assess the mineralization of each group. D Mineralization of each group was quantitatively analysed by using cetylpyridinium chloride to resolve calcium nodules, and mineralization was reflected by the absorbance of lysate at 550 nm, with the results illustrated in the bar graph. *P<0.05, compared with the NC group. #P<0.05, compared with the HG group.

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X