Fig. 1
Effects of LMHFV on the proliferation and cell cycle of HG-induced MC3T3-E1 cells. A EdU staining was performed to detect the cell proliferation of each group, and the fluorescence image of EdU-positive cells reflected the proliferation of each group. Scale bar = 100 Îĵm. B The percentage of EdU-positive cells in each group was calculated, and the results are presented in the bar graph. C The percentages of cells in G0/G1, S and G2/M phase are shown in the bar graph. D DNA content-based (stained with PI) cell cycle analysis was conducted, and the cell cycle variations of cells from the respective groups, including G0/G1, S and G2/M, were exhibited using flow cytometry histograms. *P<0.05, compared with the NC group. #P<0.05, compared with the HG group.