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Fig. 6 | Journal of Orthopaedic Surgery and Research

Fig. 6

From: Doxorubicin inhibits osteosarcoma progression by regulating circ_0000006/miR-646/ BDNF axis

Fig. 6

MiR-646 was associated with BDNF in U2OS and SJSA1 cells. A Targetscan online database was used to predict the binding sites between miR-646 and BDNF 3′UTR. B, C Dual-luciferase reporter assay was performed to detect luciferase activities in U2OS and SJSA1 cells. D, E QRT-PCR and western blot assays were carried out to reveal the mRNA and protein expression of BDNF in OS and normal bone tissues and hFOB 1.19, U2OS and SJSA1 cells, respectively. F The effect of DOX treatment on BDNF protein expression was unveiled by western blot in U2OS and SJSA1 cells. G The efficiency of BDNF overexpression was determined by western blot in U2OS and SJSA1 cells. H The influences between miR-646 mimic and BDNF overexpression on BDNF protein expression were presented by western blot. *P < 0.05

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