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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Doxorubicin inhibits osteosarcoma progression by regulating circ_0000006/miR-646/ BDNF axis

Fig. 5

Circ_0000006 silencing expedited the effects of DOX exposure on OS development via associating with miR-646. A Cell viability assay was employed to determine the effects between circ_0000006 knockdown and miR-646 inhibitor on cell viability under DOX treatment in U2OS and SJSA1 cells. B, C, E Transwell migration and wound-healing assays and transwell invasion assay were performed to illustrate the influences between circ_0000006 knockdown and miR-646 depletion on cell migration and invasion under DOX treatment, respectively, in U2OS and SJSA1 cells. D The effects between circ_0000006 knockdown and miR-646 inhibitor on cell colony-forming ability after DOX exposure were unveiled by cell colony formation assay in U2OS and SJSA1 cells. F Flow cytometry assay was carried out to disclose the impacts between circ_0000006 repression and miR-646 inhibitor on cell apoptosis under DOX exposure in U2OS and SJSA1 cells. *P < 0.05

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