Fig. 4
From: Doxorubicin inhibits osteosarcoma progression by regulating circ_0000006/miR-646/ BDNF axis
Circ_0000006 bound to miR-646 in U2OS and SJSA1 cells. A The binding sites between circ_0000006 and miR-646 were predicted by circRNA interactome online database. B, H The efficiency of miR-646 mimic and inhibitor in increasing or decreasing miR-646 expression was detected by qRT-PCR in U2OS and SJSA1 cells. C, D Dual-luciferase reporter assay was employed to determine luciferase activities in U2OS and SJSA1 cells. E, F QRT-PCR was performed to detect miR-646 expression in normal bone tissues, OS tissues and hFOB 1.19, U2OS and SJSA1 cells. G The effect of DOX treatment on miR-646 expression was illustrated by qRT-PCR in U2OS and SJSA1 cells. I QRT-PCR was carried out to demonstrate the effects between circ_0000006 knockdown and miR-646 depletion on miR-646 expression in U2OS and SJSA1 cells. *P < 0.05