Table 1 Primers used in this study
Primer | Sequence (5′-3′) |
|---|---|
miR-490-5p-RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGAC ACCCACC |
U6-RT | AACGCTTCACGAATTTGCGTG |
miR-490-5p-qPCR | Forward: TGCGGCCATGGATCTCCA Reverse: CCAGTGCAGGGTCCGAGGT |
U6-qPCR | Forward: GCTCGCTTCGGCAGCACA Reverse: GAGGTATTCGCACCAGAGGA |
Aggrecan-qPCR | Forward: AGTCCTCAAGCCTCCTGTA Reverse: CCTCCTCACATACCTCCTG |
Primer | Sequence (5′-3′) |
|---|---|
SOX9-qPCR | Forward: ACCACCAGAACTCCAGCTCCT Reverse: TCTGCGGGATGGAAGGGA |
COL2A1-qPCR | Forward: GCTCCCAGAACATCACCTACC Reverse: TGAACCTGCTATTGCCCTCT |
NONHSAT030515-qPCR | Forward: TTGGTGTTGATATGGGTA Reverse: CAAAGTTAGGAGGAAGAA |
BMPR2-qPCR | Forward: AAATAGCCTGGCAGTGAG Reverse: ATGTGACAGGTTGCGTTC |
GAPDH-qPCR | Forward: GACCTGACCTGCCGTCTA Reverse: AGGAGTGGGTGTCGCTGT |