Fig. 4

Restoring miR-331-3p counteracted the roles of circ_0045714 in IL-1β-insulted HAC. 10 ng/mL IL-1β-treated HAC cells were pre-transfected with empty vector, oe-circ_0045714 vector, or oe-circ_0045714 vector coupled with miR-NC or miR-331-3p mimic. In Control and IL-1β-treated cells, A relative miR-331-3p expression was detected by RT-qPCR, B number of the cloned cells was determined by colony formation assay, C OD values at 450 nm were measured by CCK-8 assay, D apoptosis rate was examined by apoptosis assay, E relative levels of IL-6, IL-8 and TNF-α in the culture supernatant were detected by ELISA, F, G relative expression of Bcl-2, Bax, Cleaved Casp3, ADAMTS-5, and Collagen II in cell lysate or cell culture supernatant was determined by western blotting with correction with GAPDH. *P < 0.05