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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Circ_0045714/miR-331-3p interaction affects IL-1β-evoked human articular chondrocyte injury through regulating PIK3R3 in a ceRNA regulatory cascade

Fig. 3

Circ_0045714 sponged miR-331-3p expression via target binding. A The putative miR-331-3p-binding sites and their mutations were shown in circ_0045714. B RNA expression was analyzed by RT-qPCR, and relative miR-331-3p expression was determined in miR-NC or miR-331-3p mimic-transfected HAC cells. C Luciferase activity of reporter vector was measured by dual-luciferase reporter assay, and relative luciferase activity of WT-circ_0045714 and MUT-circ_0045714 was determined in HAC cells co-transfected with miR-NC or miR-331-3p mimic. D, E RNA expression was analyzed by RT-qPCR, and relative circ_0045714 and miR-331-3p expression was determined in HAC cells transfected with oe-circ_0045714 vector, empty vector, si-circ_0045714, or si-NC. F, G RNA expression was analyzed by RT-qPCR, and relative miR-331-3p expression was determined in OA cartilages (n = 20) and chondrocytes and normal cartilages (n = 20) and chondrocytes from patients. H Linear correlation between two variables was analyzed using Pearson correlation test, and the correlation coefficient (r) between circ_0045714 and miR-331-3p in OA cartilages (n = 20) was determined. *P < 0.05

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