Fig. 2

Hyper-expression of circ_0045714 relieved HAC proliferation inhibition, apoptosis, inflammatory response, and matrix degradation under IL-1β stimulation. 10 ng/mL IL-1β-treated HAC cells were pre-transfected with oe-circ_0045714 vector or empty vector. A RNA expression was analyzed by RT-qPCR, and relative circ_0045714 expression was determined in Control and IL-1β-treated cells. B Clonal survival was measured by colony formation assay, and number of the cloned cells was determined in Control and IL-1β-treated cells. C Cell viability was evaluated by CCK-8 assay, and OD values at 450 nm were determined in Control and IL-1β-treated cells. D Cell apoptosis was examined by apoptosis assay, and apoptosis rate was determined in Control and IL-1β-treated cells. E Secretion of inflammatory factor was detected by ELISA, and relative levels of IL-6, IL-8 and TNF-α were determined in the culture supernatants of Control and IL-1β-treated cells. F, G Protein expression was analyzed by western blotting, and relative expression of F Bcl-2, Bax and Cleaved in the cell lysate, and G Casp3, ADAMTS-5 and Collagen II in the culture supernatants was determined in Control and IL-1β-treated cells with correction with GAPDH. *P < 0.05