Table 1 Primer sequences
From: MiR-4303 relieves chondrocyte inflammation by targeting ASPN in osteoarthritis
Primer name | (5′–3′) primer sequences |
|---|---|
F-miR-4303 | 5′-AGAAAATAGCTTCTGAGC-3′ |
R-miR-4303 | 5′-ACCATGGTTAGCTCCTAAGCT‐3′ |
F-ASPN | 5′-GGATTTTAAACGATACAAA-3′ |
R-ASPN | 5′-GCCTTCAGTAAATGTTCATTA‐3′ |
F-PDIA3 | 5′-ATGGGCCTGTGA AGGTAGTGG-3′ |
R-PDIA3 | 5′-TGACCACACCAA GGGGCATA-3′ |
F-PIK3CA | 5′-ATTTCATGAAACAAATGAATGATGCACA-3′ |
R-PIK3CA | 5′-CCATTTTTGTTGTCCAGCCACCATGAC-3′ |
F-TRAF3 | 5′-GCGTCGACCATGGAGTCGAGTAAAAAGA-3′ |
R-TRAF3 | 5′-TTGCGGCCGCTCAGGGATCGGGCAGATCCG-3′ |
F-U6 | 5′- CTCGCTTCGGCAGCACATATACT ‐3′ |
R-U6 | 5′-ACGCTTCACGAATTTGCGTGTC‐3′ |
F-GAPDH | 5′-GAGTCAACGGATTTGGTCGT‐3′ |
R-GAPDH | 5′-TTGATTTTGGAGGGATCTCG‐3′ |