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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: Adipogenic differentiation was inhibited by downregulation of PPARγ signaling pathway in aging tendon stem/progenitor cells

Fig. 1

Adipogenesis was inhibited in aging TSPCs. A western-blot results of senescence markers β-galactosidase and p16ink4a in young and aging TSPCs. B β-gal staining of young and aging TSPCs. C quantitative analysis of the β-gal positive staining TSPCs (%). D Oil red O staining of young and aging TSPCs after 3 weeks of adipogenic induction showed much less lipid droplets in aging group. Black bars: 50 μm. E Quantitative analysis of the lipid droplets showed significant difference between two groups. F qPCR analysis of adipogenic markers (perilipin, C/EBPα and FABP4) expression of young and aging TSPCs after 1 week of adipogenic induction showed significant difference in the expression of perilipin and FABP4. G, H western blot analysis of adipogenic markers (PPARγ, perilipin, C/EBPα and FABP4) expression of young and aging TSPCs. Expression of PPARγ, C/EBPα, FABP4 and perilipin were downregulated in aging TSPCs. I Immunofluorescence staining of adipogenic markers (Perilipin and C/EBPα) expression of young and aging TSPCs. There were more positive cells in young group both on the expression of Perilipin and C/EBPα. *p < .05; **p < .01; ***p < .001; ****p < .000. These experiments were repeated for 3 times

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