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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Recombinant TSG-6 protein inhibits the growth of capsule fibroblasts in frozen shoulder via suppressing the TGF-β/Smad2 signal pathway

Fig. 4

Recombinant TSG-6 protein restrained the growth of fibroblasts via the TGF-β/Smad2 pathway. Notes: A Western Blot was used to detect the level of TGF-β1 and phosphorylation level of Smad2 in cells. B MMT assay was employed to detect the viability of fibroblasts in each group. C, D Colony formation assay (C) and flow cytometry (D) were used to detect the proliferation and apoptosis of fibroblasts. E Western Blot was carried out to examine the levels of Bax and Bcl-2 of fibroblasts in each group. FI ELISA was used to detect the contents of COL1A1, TNF-α, IL-6, and IL-1β in the cell supernatant. J qRT-PCR was performed to determine the levels of COL1A1, TNF-α, IL-6, and IL-1β in the cells. *P < 0.05, **P < 0.01, ***P < 0.001. ELISA, enzyme-linked immunosorbent assay

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