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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Recombinant TSG-6 protein inhibits the growth of capsule fibroblasts in frozen shoulder via suppressing the TGF-β/Smad2 signal pathway

Fig. 3

Recombinant TSG-6 protein inhibited fibroblast fibrosis and inflammatory response and suppressed TGF-β/Smad2 signaling pathway. Notes: AD ELISA was used to detect the contents of COL1A1, TNF-α, IL-6, and IL-1β in the cell supernatant. E qRT-PCR was performed to determine the levels of COL1A1, TNF-α, IL-6, and IL-1β in the cells. F Western Blot was used to detect the level of TGF-β1 and phosphorylation level of Smad2 in cells. *P < 0.05, **P < 0.01, ***P < 0.001. ELISA, enzyme-linked immunosorbent assay

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